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    您當(dāng)前的位置:首頁 > 技術(shù)文章 > ELISA試劑盒絲氨酸/蘇氨酸蛋白激酶PIM3抗體分析

    ELISA試劑盒絲氨酸/蘇氨酸蛋白激酶PIM3抗體分析

    發(fā)布時間: 2014-04-24  點擊次數(shù): 848次

    ELISA試劑盒絲氨酸/蘇氨酸蛋白激酶PIM3抗體英文名稱  Anti-PIM3 
    中文名稱  絲氨酸/蘇氨酸蛋白激酶PIM3抗體 
    別    名  Kid 1; Kid-1; Kid1; Kinase induced by depolarization; ONCOGENE PIM 3; ONCOGENE PIM3; PIM 3; Pim 3 oncogene; PIM3; Pim3 oncogene; PIM3_HUMAN; Protein kinase Kid 1; Protein kinase Kid1; Serine/threonine kinase Pim 3; Serine/threonine kinase Pim3; Serine/threonine protein kinase Pim 3; Serine/threonine protein kinase Pim3; Serine/threonine-protein kinase Pim-3.  
    濃    度  1mg/1ml 
    規(guī) 格  0.2ml/200μg 
    抗體來源  Rabbit  
    克隆類型  polyclonal 
    交叉反應(yīng)  Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig
    產(chǎn)品類型  一抗    
    ELISA試劑盒絲氨酸/蘇氨酸蛋白激酶PIM3抗體研究領(lǐng)域  腫瘤 細胞生物 信號轉(zhuǎn)導(dǎo) 激酶和磷酸酶  
    蛋白分子量  predicted molecular weight: 36kDa 
    性    狀  Lyophilized or Liquid 
    免 疫 原  KLH conjugated synthetic peptide derived from human PIM3 
    亞    型  IgG 
    純化方法  affinity purified by Protein A 
    儲 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 
    產(chǎn)品應(yīng)用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:50-200 
    (石蠟切片需做抗原修復(fù)) 
     not yet tested in other applications.
     optimal dilutions/concentrations should be determined by the end user.  
    保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
    Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
    ELISA試劑盒絲氨酸/蘇氨酸蛋白激酶PIM3抗體產(chǎn)品介紹 The Pim family serine/threonine protein kinases were first identified in studies examining genes targeted for proviral insertion in murine leukemia virus-induced T lymphomas. Increased levels of Pim kinases predispose cells to lymphomagenesis and enhance the activity of mitogenic proteins such as p100, c-Myb, and Cdc2. In addition, Pim kinases are also involved in modulation of synaptic strength in neurons and anti-apoptotic signaling in hematopoietic progenitor cells. Pim-3, a member of the proto-oncogene Pim family that expresses serine/threonine kinase activity, shares significant homology with Pim-1 serine/threonine protein kinases. Pim-3 may function as a mediator of synaptic plasticity in the brain and is presumably involved in the anti-apoptosis process and cell cycle progression as well as the proliferation of human hepatoma cell lines. The Pim-3 protein is widely expressed, however no expression is observed in the colon, thymus, or small intestine.
    Function : Proto-oncogene with serine/threonine kinase activity that can prevent apoptosis, promote cell survival and protein translation. May contribute to tumorigenesis through: the delivery of survival signaling through phosphorylation of BAD which induces release of the anti-apoptotic protein Bcl-X(L), the regulation of cell cycle progression, protein synthesis and by regulation of MYC transcriptional activity. Additionally to this role on tumorigenesis, can also negatively regulate insulin secretion by inhibiting the activation of MAPK1/3 (ERK1/2), through SOCS6. Involved also in the control of energy metabolism and regulation of AMPK activity in modulating MYC and PPARGC1A protein levels and cell growth.
    Subunit : Interacts with BAD. Interacts with PPP2CA; this interaction promotes dephosphorylation of PIM3, ubiquitination and proteasomal degradation. Interacts with SOCS6 (By similarity).
    Subcellular Location : Cytoplasm.
    Tissue Specificity : Detected in various tissues, including the heart, brain, lung, kidney, spleen, placenta, skeletal muscle, and peripheral blood leukocytes. Not found or barely expressed in the normal adult endoderm-derived organs such as colon, thymus, liver, or small intestine. However, expression is augmented in premalignant and malignant lesions of these organs.
    Post-translational modifications : Ubiquitinated, leading to proteasomal degradation.
    Phosphorylated. Interaction with PPP2CA promotes dephosphorylation.
    Similarity : Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
    Contains 1 protein kinase domain.
    Database links : 
    UniProtKB/Swiss-Prot: Q86V86.3 
    Entrez Gene: 415116 Human 
    Entrez Gene: 223775 Mouse 
    Omim: 610580 Human 
    SwissProt: Q86V86 Human 
    SwissProt: P58750 Mouse 
    Unigene: 530381 Human 
    Unigene: Hs.530381 Human 
    Unigene: 400129 Mouse 

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